prot: GROUP ATOMS=1-174

WHOLEMOLECULES ENTITY0=prot

cs: CS2BACKBONE ATOMS=prot FF=a03_gromacs.mdb DATA=data/ CYS-DISU TERMINI=DEFAULT,NONE NRES=13 FLAT=0.0 ENSEMBLE

cse: RESTRAINT ARG=cs SLOPE=4.0 KAPPA=0. AT=0.
#increse it slowly to the target value (at ~100ps per increse step)
#cse: RESTRAINT ARG=cs SLOPE=8.0 KAPPA=0. AT=0.
#cse: RESTRAINT ARG=cs SLOPE=12.0 KAPPA=0. AT=0.
#cse: RESTRAINT ARG=cs SLOPE=16.0 KAPPA=0. AT=0.
#cse: RESTRAINT ARG=cs SLOPE=20.0 KAPPA=0. AT=0.
#cse: RESTRAINT ARG=cs SLOPE=24.0 KAPPA=0. AT=0.

PRINT STRIDE=10 ARG=cs,cse.bias FILE=COLVAR

FLUSH STRIDE=10 

ENDPLUMED
